Intracellular conformations of human telomeric quadruplexes studied by electron paramagnetic resonance spectroscopy.

نویسندگان

  • Mykhailo Azarkh
  • Vijay Singh
  • Oliver Okle
  • Daniel R Dietrich
  • Jörg S Hartig
  • Malte Drescher
چکیده

In-cell DEER (in-cell double electron electron resonance) is applied to investigate quadruplex formation of the human telomeric repeat d[AGGG(TTAGGG)(3)]. The initially unfolded DNA sequence forms a mixture of different quadruplex topologies upon injection into living cells. In addition, time-dependent distance measurements are carried out to monitor quadruplex folding.

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عنوان ژورنال:
  • Chemphyschem : a European journal of chemical physics and physical chemistry

دوره 13 6  شماره 

صفحات  -

تاریخ انتشار 2012